Dual priming oligonucleotide (DPO)-based multiplex PCR assay for specific detection of four diarrhoeagenic Escherichia coli in food
نویسندگان
چکیده
منابع مشابه
Use of multiplex PCR assay for detection of diarrheagenic Escherichia coli in street vended food items
Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with a...
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Acute diarrhoeal disease is a public health problem and an important cause of morbidity and mortality, particularly in developing countries. The etiology is varied, and the diarrheagenic Escherichiacolipathotypes are most important. Our objectives were detection of Diarrhoeagenic E. coli virulence genes by Multiplex-PCR method and their antimicrobial susceptibility profile. Fifty five E. Coli s...
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BACKGROUND Exon deletions of Duchenne muscular dystrophy (DMD) gene account for most of the alterations found in DMD and Becker muscular dystrophy (BMD). This study was to evaluate the usefulness of dual priming oligonucleotide multiplex PCR (DPO PCR) in detection of exon deletions of DMD gene. METHODS Thirty-seven DMD or BMD patients who had known exon deletions detected by conventional mult...
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BACKGROUND A cost-effective, accurate and rapid simultaneous multiplex assay is required for testing and diagnoses of conventional and emerging viruses in clinical virology laboratories. We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1. METHODS The optimiz...
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A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2015
ISSN: 0266-8254
DOI: 10.1111/lam.12426